Journal: GeroScience

Article Title: Circulating interleukin-37 declines with aging in healthy humans: relations to healthspan indicators and IL37 gene SNPs

doi: 10.1007/s11357-022-00587-3

Figure Lengend Snippet: Validation of the AdipoGen interleukin (IL)-37 ELISA. A Measured vs. actual concentrations of human IL-37 recombinant protein were very similar. B IL-37 measured in human plasma is reproducible across days and assay kits ( n = 8). C Concentrations of IL-37 were higher in plasma than in peripheral blood mononuclear cell (PBMC) lysates from the same subjects ( n = 10; data are mean ± SD with individual data). D Adding IL-1 receptor antagonist (IL-1ra) to assay buffer across a range of IL-37 concentrations did not affect IL-37 measured by the ELISA, confirming specificity for IL-37 ( n = 9/condition). E IL-37 concentrations were similar between plasma and serum from the same subjects ( n = 17)

Article Snippet: As such, we conducted experiments to validate the accuracy and specificity of the AdipoGen IL-37 ELISA, as well as whether it can be used reliably in human plasma samples.

Techniques: Enzyme-linked Immunosorbent Assay, Recombinant

Journal: GeroScience

Article Title: Circulating interleukin-37 declines with aging in healthy humans: relations to healthspan indicators and IL37 gene SNPs

doi: 10.1007/s11357-022-00587-3

Figure Lengend Snippet: Circulating concentrations of interleukin (IL)-37 are reduced with aging and insufficient to balance age-related increases in pro-inflammatory markers. Plasma IL-37 ( A ) and the ratios of IL-37 to pro-inflammatory serum C-reactive protein ( B ) and IL-6 ( C ) in young (Y; 18–39 years; n = 34–38), middle-aged (MA; 40–64 years; n = 130–145), and older (O; 65 + years; n = 51–61) adults. Outliers for each marker were removed using ROUT ( Q = 0.1%); subjects included in ratios if data for both markers remained after outlier removal. Graphs are mean ± SD with individual data. * P < 0.05 vs. young adults. † P < 0.05 vs. middle-aged adults. b P < 0.10 vs. middle-aged adults (one-way ANOVA with Tukey’s post hoc test)

Article Snippet: As such, we conducted experiments to validate the accuracy and specificity of the AdipoGen IL-37 ELISA, as well as whether it can be used reliably in human plasma samples.

Techniques: Marker

Journal: GeroScience

Article Title: Circulating interleukin-37 declines with aging in healthy humans: relations to healthspan indicators and IL37 gene SNPs

doi: 10.1007/s11357-022-00587-3

Figure Lengend Snippet: Significant correlations between healthspan markers and the ratios of plasma interleukin (IL)-37 and C-reactive protein (CRP) or IL-6 in young (18–39 years; n = 34–38), middle-aged (40–64 years; n = 130–145), and older (65 + years; n = 51–61) adults. Regression coefficients and P values shown are the results of unadjusted Pearson correlations. Only statistically significant correlations ( P < 0.05) are shown

Article Snippet: As such, we conducted experiments to validate the accuracy and specificity of the AdipoGen IL-37 ELISA, as well as whether it can be used reliably in human plasma samples.

Techniques:

Journal: GeroScience

Article Title: Circulating interleukin-37 declines with aging in healthy humans: relations to healthspan indicators and IL37 gene SNPs

doi: 10.1007/s11357-022-00587-3

Figure Lengend Snippet: Genotypes for SNPs in IL37 and ILR8 do not predict plasma concentrations of IL-37 protein. Plasma IL-37 protein concentrations in individuals with 0, 1, or 2 copies of the minor allele in A HAP1 in the IL37 gene (rs3811046; minor allele: G); B HAP2 in the IL37 gene (rs2723192; minor allele: C); and C the ILR8 HAP (rs7947; minor allele: G). Plasma IL-37 protein concentrations are log-transformed to account for skewness (outliers excluded; ROUT: Q = 1%). Graphs are mean ± SD with individual data. The final numbers of subjects included in this analysis with 0, 1, and 2 copies, respectively, of each haplotype are as follows: n = 91, 81, and 15 for HAP1 in the IL37 gene; n = 166, 26, and 3 for HAP2 in the IL37 gene; and n = 116, 69, and 10 for the ILR8 HAP

Article Snippet: As such, we conducted experiments to validate the accuracy and specificity of the AdipoGen IL-37 ELISA, as well as whether it can be used reliably in human plasma samples.

Techniques: Transformation Assay

Journal: GeroScience

Article Title: Circulating interleukin-37 declines with aging in healthy humans: relations to healthspan indicators and IL37 gene SNPs

doi: 10.1007/s11357-022-00587-3

Figure Lengend Snippet: IL-37 in the context of biological aging. Chronic low-grade inflammation and immunosenescence are two hallmark mechanisms of biological aging. In the present study, circulating concentrations of IL-37 were lower in healthy older adults, compared to young and middle-aged adults, which may both reflect immunosenescence (i.e., impaired ability to mount an anti-inflammatory response) and contribute to chronic low-grade inflammation (i.e., reduced suppression of production of pro-inflammatory cytokines). Importantly, reduced ratios of IL-37 to key pro-inflammatory markers were associated with impairments in several markers of healthspan, suggesting that both pro-inflammatory and anti-inflammatory processes should be considered in future studies and models of biological aging and healthspan

Article Snippet: As such, we conducted experiments to validate the accuracy and specificity of the AdipoGen IL-37 ELISA, as well as whether it can be used reliably in human plasma samples.

Techniques: